Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum

Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum

To the Editor: An outbreak of rec-tal lymphogranuloma venereum (LGV) has been detected in the Netherlands among men who have sex with men (1–4). More cases of LGV in other European countries such as Belgium, France, and the United Kingdom have been reported, and the first cases have been detected in the United States as well. This infection is encountered not only by clinicians who treat sexual...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Real-time polymerase chain reaction.

Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...

Polymerase Chain Reaction to Diagnose Infective Endocarditis

report on a series of 52 excised heart valves on which they performed broad-range polymerase chain reaction (PCR) to identify microbes in valve tissues that were defined as having infective endocarditis (IE). Internal sequencing of amplicons, with specific “nested” primers to identify microbial subspecies, was performed after broad-range PCR. The authors report that on the basis of gross featur...

A multiplex real-time polymerase chain reaction assay to diagnose Epiphyas postvittana (Lepidoptera: Tortricidae).

A molecular assay for diagnosis of light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), in North America is reported. The assay multiplexes two TaqMan real-time polymerase chain reaction (RT-PCR) probe systems that are designed to target DNA segments of the internal transcribed spacer region 2 (ITS2) and 18S rRNA gene. The RT-PCR probe designed for the 18S target re...