Real-Time Polymerase Chain Reaction Assay for the Diagnosis of Pulmonary Tuberculosis

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Real-Time Polymerase Chain Reaction Assay for the Diagnosis of Pulmonary Tuberculosis

1. Kim SW, Kim SI, Lee SJ, Lee JH, Ryu YJ, Shim SS, et al. The effectiveness of real-time PCR assay, compared with microbiologic results for the diagnosis of pulmonary tuberculosis. Tuberc Respir Dis 2015;78:1-7. 2. Kheawon N, Chuang-Ngam S, Mitsoongneun S, Peam-Am J, Visalsawadi J. Sensitivity and specificity of real time polymerase chain reaction (RT-PCR) in bronchial washing for diagnostic p...

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...

polymerase chain reaction for the diagnosis of tuberculosis

dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*