Real time PCR quantification of Sclerotium rolfsii in chilli tissue and soil
نویسندگان
چکیده
منابع مشابه
Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
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In this study, we characterized sporadically occurring sclerotium rot caused by Sclerotium rolfsii in Chinese chive (Allium tuberosum Roth.) in farm fields in Sacheon, Korea. The initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. Further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the sca...
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The root-lesion nematode Pratylenchus thornei is one of the most important pests restricting productivity of wheat in the Pacific Northwest (PNW). It is laborious and difficult to use microscopy to count and identify the nematodes in soils. A SYBR Green I-based real-time polymerase chain reaction (PCR) assay was developed to detect and quantify this species from DNA extracts of soil. A primer s...
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A real-time PCR assay was developed to quantify in soil the fungus Hirsutella minnesotensis, an important parasite of secondary-stage juvenile (J2) of the soybean cyst nematode. A primer pair 50-GGGAGGCCCGGTGGA-30 and 50-TGATCCGAGGTCAACTTCTGAA-30 and a TaqMan probe 50-CGTCCGCCGTAAAACGCCCAAC-30 were designed based on the sequence of the ITS region of the rRNA gene. The primers were highly specie...
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ژورنال
عنوان ژورنال: Plant Protection Science
سال: 2016
ISSN: 1212-2580,1805-9341
DOI: 10.17221/43/2014-pps