منابع مشابه
Real-Time PCR: An Essential Guide
Real-time polymerase chain reaction (PCR) technique has advanced greatly over the past 10 years. This timely, comprehensive publication includes information on currently available instrumentation, fluorescent chemistries, assay design, optimization, and validation strategies. The background chapters are followed by chapters on quantification, single nucleotide polymorphism (SNP), mutation detec...
متن کاملReal-Time PCR: An Essential Guide
Chapter abstracts How to Order Real-time PCR (RT-PCR) is a powerful and rapid technique for nucleic acid amplification. The accumulation of specific products in a reaction is monitored continuously during cycling. This is usually achieved by monitoring changes in fluorescence within the PCR tube. This essential manual presents a comprehensive guide to the most appropriate and up-to-date technol...
متن کاملAn Overview of Real-Time PCR Platforms
Real-time PCR continues to have a major impact across many disciplines of the biological sciences and this has been a driver to develop and improve existing instruments. From the fi rst two commercial platforms introduced in the mid 1990s, there is now a choice in excess of a dozen instruments, which continues to increase. Advances include faster thermocycling times, higher throughput, fl exibi...
متن کاملAn Introduction to Real-Time PCR
The development of instruments that allowed real-time monitoring of fl uorescence within PCR reaction vessels was a very signifi cant advance. The technology is very flexible and many alternative instruments and fl uorescent probe systems have been developed and are currently available. Real-time PCR assays can be completed very rapidly since no manipulations are required post-amplifi cation. I...
متن کاملReal-Time PCR Platforms
Real-time PCR continues to have a major impact across many disciplines of the biological sciences and this has been a driver to develop and improve existing instruments. From the first two commercial platforms introduced in the mid 1990s, there is now a choice in excess of a dozen instruments, which continues to increase. Advances include faster thermocycling times, higher throughput, flexibili...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2004
ISSN: 1460-2091,0305-7453
DOI: 10.1093/jac/dkh439