Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation

Optimization of Real Time PCR for Precise Measurement of HER2 Overexpression in Breast Cancer Specimens

Background: Breast cancer is one of the most prevalent malignancies among women in various countries. HER2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. HER2 gene encodes an 185kDa transmembrane glycoprotein with 1255 amino acids. This active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. These...

Antibody-Mediated Antigen Presentation to T and Antigen Determines the Efficiency of The Rate of Dissociation Between Antibody

Determination of thermodynamic parameters of antigen–antibody interaction from real-time kinetic studies

Real-time kinetic parameters of the interaction of iodinated human chorionic gonodotropin (IhCG) with one of its monoclonal antibodies are sought and are determined after an analysis of the dissociation profile of IhCG–MAb (monoclonal antibody) complexes in the presence of excess unlabelled hCG at various temperatures. The equilibrium association constant (KA) of the epitope–paratope reaction (...

Calcium-dependent antigen binding as a novel modality for antibody recycling by endosomal antigen dissociation

The pH-dependent antigen binding antibody, termed a recycling antibody, has recently been reported as an attractive type of second-generation engineered therapeutic antibody. A recycling antibody can dissociate antigen in the acidic endosome, and thus bind to its antigen multiple times. As a consequence, a recycling antibody can neutralize large amounts of antigen in plasma. Because this approa...

Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation

Nonexcitable cell types, fibroblasts of heart muscle or astrocytes, are well known for their spontaneous Ca(2+) oscillations. On the other hand, murine fibroblast (L929) cells are known to be deficient in cell-cell adhesive proteins and therefore lack gap junctions for cellular communication. However, these cells exhibit a unique property of collectively synchronized and spontaneous oscillation...