Reactivities of Actin as a Contractile Protein

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Reactivities of Actin as a Contractile Protein

The molecular basis for the mechanism of contraction in striated muscle, with primary emphasis on the interaction between the thick and thin filaments and the role of the thin (actin) filaments, is the theme presented. Recent information relating to actin-myosin interaction points up the fact that definitive statements cannot be made regarding the molecular interaction(s) that lead to contracti...

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Reassembly of contractile actin cortex

The contractile cortex is a 50-nm–2-μm-thick layer of cytoskeleton under the plasma membrane that is rich in actin fi laments, myosin II, and actin-binding proteins (Bray and White, 1988). Assembly dynamics and contractility of this layer are thought to generate cortical tension, drive cytokinesis, and play a central role in cell locomotion and tissue morphogenesis (Bray and White, 1988; Albert...

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Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein

Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...

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production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1967

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.50.6.119