Ratio quantification of gene dosage by Agilent 2100 Bioanalyzer for detection of somatic gene deletions

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection of a point mutation in the prothrombin gene with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer can be used for the detection of a point mutation in the human prothrombin gene. The prothrombin protein plays a key role in blood clotting. A single nucleotide polymorphism (SNP), i.e. a point mutation in the prothrombin gene results in a common hereditary predisposition to venous thrombosis. We present a convenient and reliable ...

متن کامل

Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene.

The Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, Calif.) utilizes capillary electrophoresis on a microchip device (LabChip 7500; Caliper Technologies, Mountain View, Calif.) that is capable of rapidly sizing small DNA fragments. To determine whether the system could replace conventional restriction fragment length polymorphism (RFLP) typing by agarose gel electrophoresis, we compa...

متن کامل

Analysis of small RNAs with the Agilent 2100 Bioanalyzer

Isolation of pure and intact RNA is essential, but the concentration and integrity of total RNA preparations is affected by the purification process used. Applications for which RNA will ultimately be used may include reverse transcriptase (RT)-PCR, preparations of targets for microarrays, ribonuclease protection assays, preparations of cDNA libraries and northern blotting. Recently, the import...

متن کامل

Analysis of small RNAs with the Agilent 2100 bioanalyzer

The Total RNA 6000 Nano Kit was used with the Agilent 2100 bioanalyzer to determine the integrity and the concentration of small RNA (i.e., 5.8S, 5S RNAs and tRNA) samples extracted with different protocols from various cell lines. The small RNAs fraction (<200 bp) should also contain microRNAs in their primitive (pri-miRNA), precursor (premiRNA) and mature (miRNA) forms. The data analysis soft...

متن کامل

Detection of DNA mutations associated with mitochondrial diseases by Agilent 2100 bioanalyzer.

BACKGROUND Molecular analysis of mitochondrial DNA (mtDNA) has provided a final diagnosis for many of the mitochondrial diseases. We evaluated the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) to determine whether the system could replace the conventional restriction fragment length polymorphism (RFLP) analysis by the agarose gel electrophoresis for the detection of the mtDNA m...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Advances in Biological Chemistry

سال: 2012

ISSN: 2162-2183,2162-2191

DOI: 10.4236/abc.2012.21009