Rapid synthesis of cDNA for cloning into lambda vectors
نویسندگان
چکیده
منابع مشابه
Molecular cloning of a bovine immunoglobulin lambda chain cDNA.
A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) lambda light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig lambda chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) se...
متن کاملCloning PCR products into T vectors.
MATERIALS Bacteriophage T4 DNA ligase T vector Target DNA (25 μg/ml), amplified by PCR When the PCR mixture contains more than one or two bands of amplified DNA, purify the target fragment by electrophoresis through low melting/gelling temperature agarose (please see Recovery of DNA from Low-meltingtemperature Agarose Gels: Organic Extraction). If not purified by gel electrophoresis, PCR-amplif...
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Name of Sequence. Bovine serum amyloid A3. Genus and Species, Breed. Bos taurus, Holstein. Origin of Clone. cDNA clones that showed differentially up-regulated message in involuted mammary tissues compared with lactating tissues have been isolated by a subtractive suppression hybridization method. These clones were sequenced by a dideoxy method using ABI PRISM Big Dye Terminators (Forster City,...
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We have used a biotin-based subtraction procedure to enrich a potato tuber cDNA library for sink-specific clones. The method uses single-stranded phagemids with directional inserts as both driver and target. We modified the XZAP II vector for the directional cDNA cloning and for subsequent subtractive hybridization. This improved method of subtractive hybridization circumvents many problems fac...
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Human and chimpanzee adenovirus vectors are being developed to circumvent preexisting antibodies against common adenovirus vectors such as Ad5. However, baseline immunity to these vectors still exists in human populations. Traditional cloning of new adenovirus vaccine vectors is a long and cumbersome process that takes 2 months or more and that requires rare unique restriction enzyme sites. Her...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.4.1913