Rapid staining technique for Trichomonas vaginalis. Preliminary report.
نویسندگان
چکیده
منابع مشابه
Modified Field's staining--a rapid stain for Trichomonas vaginalis.
Trichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract, is transmitted primarily by sexual intercourse. Diagnosis is usually by in vitro culture method and staining with Giemsa stain. There are laboratories that use Gram stain as well. We compared the use of modified Field's (MF), Giemsa, and Gram stains on 2 axenic and xenic isolates of T. vagina...
متن کاملSimple staining method for Trichomonas vaginalis.
In a recent annotation in this Journal (1952) it was pointed out that before Trichomonas vaginalis could be identified with certainty in stained films the flagellar structure had to be demonstrated, and that successful staining of this structure depended upon careful fixation of the film. This need for careful fixation appears to be the main reason why only experts obtain uniformly good results...
متن کاملRapid assay for immunological detection of Trichomonas vaginalis.
Trichomoniasis is a common sexually transmitted disease with an estimated incidence of 4 million to 8 million cases a year in the United States. The most commonly used method of diagnosis is a direct microscopic observation (wet mount) of vaginal secretions and, although both rapid and inexpensive, the sensitivity of this technique is generally 50 to 70%. We developed an indirect enzyme-linked ...
متن کاملTrichomonas vaginalis
We have investigated the effects of a novel neutrophil-activating factor released by Trichomonas vaginalis (TV-NAF) on neutrophil chemotaxis. TV-NAF was present in the supernatant from 107 T. vaginalis (STV) cultured in 1 ml of serum-free Hanks' balanced salt solution (HBSS) at 37°C for 30 min. With a multichamber chemotactic assay, we found that there were 112 + 15 migrated neutrophils (mean ±...
متن کاملA preliminary investigation of microsatellite-based genotyping in Trichomonas vaginalis.
The genetic epidemiology of Trichomonas vaginalis is poorly understood at present. The recent release of the organism's genome sequence opens the way to investigation of polymorphic markers allowing strain identification. We here report a preliminary analysis of microsatellite loci in T. vaginalis and show that this approach holds promise for future studies of infection transmission and organis...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1979
ISSN: 1368-4973
DOI: 10.1136/sti.55.4.289