Rapid restriction analysis of YAC clones
نویسندگان
چکیده
منابع مشابه
Method for rapid restriction analysis of YAC clones.
The development of the yeast artificial chromosome (YACs) technology (3) has provided, by virtue of its capacity to carry large fragments of genomic DNA, the possibility of linking the genetic map and the physical map of an organism. This requires the alignment of overlapping YAC clones into contigs for which a number of strategies have been described as follows: the use of sequence tagged site...
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The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA frag...
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To date, frozen storage of YAC libraries have relied on the administration of glycerol to the medium subsequent to cell growth. By adding Hogness Freezing Medium prior to inoculation, cultures can be frozen directly after cell growth, with no adverse effect on the stability of the YAC DNA or on the viability of the cells even after repeated freezing and defrosting. Although a relatively simple ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.7.1318