Rapid, Quantitative PCR Monitoring of Growth of Clostridium botulinum Type E in Modified-Atmosphere-Packaged Fish
نویسندگان
چکیده
منابع مشابه
Rapid, quantitative PCR monitoring of growth of Clostridium botulinum type E in modified-atmosphere-packaged fish.
A rapid, quantitative PCR assay (TaqMan assay) which quantifies Clostridium botulinum type E by amplifying a 280-bp sequence from the botulinum neurotoxin type E (BoNT/E) gene is described. With this method, which uses the hydrolysis of an internal fluoregenic probe and monitors in real time the increase in the intensity of fluorescence during PCR by using the ABI Prism 7700 sequence detection ...
متن کاملDouble Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E
Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA m...
متن کاملIsolation of Clostridium botulinum type E from Cayuga Lake fish.
The vast majority of the isolations of Clostridium botulinum type E have been from marine fish and their environment, with very few from fresh-water lakes and streams. Johannsen (J. Appl. Bacteriol. 26:43, 1963) has found the organisms to be common in Norway and Sweden's coastal and river waters where the salinity is fairly low, and Prev6t and Huet (Bull. Acad. Natl. Med., Paris 135:432, 1951) ...
متن کاملGrowth and toxigenesis behavior of Clostridium botulinum type E in Persian sturgeon (Acipenser persicus) Caviar prepared with various preservatives
Growth behavior of Clostridium botulinum type E beluga was studied in Acipenser persicus granular caviar treated with 5% NaCl, 5%NaCl plus 0.3% boric acid and 0.4%borax and 5%NaCl plus 0.15% methyl paraben incubated at temperatures -2, 5 and 15 °C for 224 days. The initial number of inoculated bacterial spore was 5.1×104 cfu/g caviar in each treated sample. The number of bacterial spores in sam...
متن کاملPrevalence, Molecular Epidemiology and Growth of Clostridium Botulinum Type E in Fish and Fishery Products
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2001
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.67.1.206-216.2001