Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN

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Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN.

We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes...

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A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.

Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at...

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Detection of cytomegalovirus from blood leukocytes separated by sepracell-MN and Ficoll-Paque/Macrodex methods.

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Ficoll-Hypaque separation of bone marrow cells.

Levitt et aP and Greenberg et al’ have recently described elegant immunologic methodologies for selectively isolating T lymphocytes’ or hematopoietic progenitor cells2 from small aliquots of human marrow. In both cases the initial marrow manipulation, following aspiration, involved selective isolation of light-density mononuclear cells using Ficoll-Hypaque (I .077 ± 0.003 g/dL) gradients. Poten...

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Use of colloidal silica (Sepracell-MN) for enrichment of dendritic cells from human peripheral blood: comparison with other methods.

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ژورنال

عنوان ژورنال: Journal of Immunological Methods

سال: 1988

ISSN: 0022-1759

DOI: 10.1016/0022-1759(88)90104-4