Rapid preparation of DNA dot blots from tissue samples, using hot alkaline lysis and filtration onto charge-modified nylon membrane
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چکیده
منابع مشابه
Improved alkaline lysis method for rapid isolation of plasmid DNA.
Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...
متن کاملDot and slot blotting of DNA.
Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a hybridization pattern that is amenable to analysis...
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1.Josefsen, K., J.P. Stenvang, H. Kindmark, P.O. Berggren, T. Horn, T. Kjaer and K. Buschard. 1996. Fluorescence-activated cell sorted rat islet cells and studies of the insulin secretory process. J. Endocrinol. 149:145-154. 2.Kannourakis, G., G.R. Johnson and F. Battye. 1988. Clonal proliferation in vitro of individual murine and human hemopoietic cells after fluorescence-activated cell sortin...
متن کاملExtraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.
We found that with our assay conditions, TCEP comletely reduced the GSSG that was present within the amples to GSH. Furthermore, the presence of TCEP id not interfere with the labeling of GSH by mBrB nor ith the detection of the glutathione–bimane product. his was true for samples consisting of either purified lutathione or acidified extracts of whole blood. As vidence for this, when samples po...
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NOVEMBER 2005 P ositioned at various stages during bioprocessing, depth filters are used as prefilters that clarify fluid streams to protect, prolong the life, and increase the efficiency of final (sterilizing) filters, chromatography columns, and other separation technologies. Depth filters are particularly well-suited for use in prefiltration during bioprocessing, removing large contaminants ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.10.3093