Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing
نویسندگان
چکیده
منابع مشابه
Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.
The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonu...
متن کاملThermonuclease test for same-day identification of Staphylococcus aureus in blood cultures.
We used a thermonuclease test to presumptively identify Staphylococcus aureus directly in blood cultures on the same day that a smear of the broth showed gram-positive cocci in clusters. There were no discrepancies between the identification of S. aureus directly from 250 blood cultures and identification by the tube coagulase test which was performed on the 18- to 24-h subculture isolates. The...
متن کاملRapid identification of Staphyloccocus aureus in positive-testing blood cultures by Slidex Staph Plus agglutination test.
In two recent articles in the Journal of Clinical Microbiology, Qian et al. (4) and Lagace-Wiens et al. (2) describe a direct coagulase and a thermostable DNase test, respectively, for direct identification of Staphylococcus aureus from positive blood culture bottles. In a prospective study from April 2003 until February 2004, we investigated the accuracy of the Slidex Staph Plus agglutination ...
متن کاملEvaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures.
Staphylococci represent the most commonly encountered blood culture isolates. Differentiating Staphylococcus aureus from coagulase-negative staphylococci (CoNS) is important in guiding empirical therapy, especially since the majority of CoNS are contaminants. This study evaluated three rapid methods for the direct identification of S. aureus from blood cultures. A total of 157 patient blood cul...
متن کاملProblems of thermonuclease detection for identifying Staphylococcus aureus in blood culture broths.
The detection of thermonuclease by the Oxford strain and eight clinical isolates of Staphylococcus aureus in a variety of bacteriological broths with and without added blood was examined using a toluidine blue-DNA-agar plate method. In Isosensitest, brain-heart infusion, tryptic soy, nutrient and gas-liquid chromatography broths (all of which do not contain liquoid) thermonuclease detection was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1983
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.18.3.722-724.1983