Rapid identification of clinical Trichoderma longibrachiatum isolates by cellulose-acetate electrophoresis-mediated isoenzyme analysis
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چکیده
منابع مشابه
Rapid cellulose acetate electrophoresis. I. Serum proteins.
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
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An immunoelectrophoretic procedure utilizing microprecipitation and cellulose acetate electrophoresis was developed for detection of antibody to specific virus. A model system of tobacco mosaic virus and homologous rabbit antiserum is described.
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We present a new method for quantitative determination of alkaline phosphatase isoenzymes. This method consists of electrophoretic separation on cellulose acetate membranes, special fixation technique to avoid elution and diffusion of enzyme protein during incubation, specific staining, and quantitative evaluation by densitometric measurement. We highly recommend the precedure for routine clini...
متن کاملRapid electrophoresis and quantitation of haemoglobins on cellulose acetate.
A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...
متن کاملBRIEF REPORT Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates
I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have propose...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2006
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2005.01356.x