Rapid Construction of Sequencing Templates by Random Insertion of Antibiotic Resistance Genes
نویسندگان
چکیده
منابع مشابه
Rapid construction of sequencing templates by random insertion of antibiotic resistance genes.
We describe a novel and handy method for generating a population of templates for sequencing. The method is based on the random insertion of antibiotic resistance gene in plasmid DNA digested by DNase I. The advantages of this approach are the small quantity of DNA necessary for mutagenesis and the complete independence from the restriction map of the plasmid. DNase I digestion provides a rando...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02322st04