Rapid Chair-Side Test for Detection of Porphyromonas gingivalis

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Development and evaluation of a saliva-based chair-side diagnostic for the detection of Porphyromonas gingivalis

Porphyromonas gingivalis is a key pathogen in the polymicrobial biofilm that is associated with the oral disease chronic periodontitis. A number of studies have shown that in humans the level of P. gingivalis in the polymicrobial biofilm is positively correlated with disease progression. The aim of this study was to develop a P. gingivalis diagnostic that has high specificity and sensitivity fo...

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Article Type: Full Length Research Article Polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP) represent a major advance in diagnostic methods in terms of speed and sensitivity. However, standard methods for sample preparation prior to PCR and LAMP are laborious and time consuming. Three template preparation methods (crude template, boiling, and commercial DNA extr...

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Porphyromonas gingivalis strain diversity.

Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Genotyping studies suggest that genetic variability exists among P. gingivalis strains; however, the extent of variability remains unclear and regions of variability remain largely unidentified. To assess P. gingivalis strain diversity, we previously used heteroduplex analysis of the ribosomal operon intergenic spa...

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ژورنال

عنوان ژورنال: Journal of Dental Research

سال: 2017

ISSN: 0022-0345,1544-0591

DOI: 10.1177/0022034517691720