منابع مشابه
Rapid 3D light-sheet microscopy with a tunable lens.
The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the f...
متن کاملHyperspectral light sheet microscopy
To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varyin...
متن کاملLight Sheet Module Applied in 3d Live Cell Microscopy
Innovative fiber-coupled add-ons for light sheet-based fluorescence microscopy (LSFM) with commercial inverted microscopes were described previously [1]. The light sheet is generated either by an achromatic cylindrical lens or by a spherical mirror with astigmatic distortion. Presently the “lens system” is further validated and applied to various samples including 3D multicellular spheroids and...
متن کاملConfocal multiview light-sheet microscopy.
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging w...
متن کاملLight-sheet optimization for microscopy
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM)...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2013
ISSN: 1094-4087
DOI: 10.1364/oe.21.021010