منابع مشابه
Running on Ran Nuclear Transport and the Mitotic Spindle
sol and GTP hydrolysis. In particular, nuclear import Far from being passive participants in the process of is accomplished for proteins bearing classical nuclear mitosis, chromosomes have an active and essential role localization signals (NLS) by a heterodimeric receptor in the assembly of spindles required for their accurate comprised of a Ran-GTP binding protein, importin b, division into th...
متن کاملNuclear transport factor p10/NTF2 functions as a Ran–GDP dissociation inhibitor (Ran-GDI)
The cytosolic nuclear transport factor p10/NTF2 is required for the translocation of karyophilic molecules through nuclear pores [1] [2] [3], and the small GTPase Ran is a key regulator of protein transport between the nucleus and cytoplasm [4] [5]. It has been reported that p10/NTF2 interacts directly and specifically with Ran-GDP but not with Ran-GTP [6]. The precise role(s) of p10/NTF2 in th...
متن کاملSystems analysis of Ran transport.
The separate components of nucleocytoplasmic transport have been well characterized, including the key regulatory role of Ran, a guanine nucleotide triphosphatase. However, the overall system behavior in intact cells is difficult to analyze because the dynamics of these components are interdependent. We used a combined experimental and computational approach to study Ran transport in vivo. The ...
متن کاملHigh-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport.
High-resolution scanning electron microscopy provides three-dimensional surface images of nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we describe a method for exposing the nuclear surface in mammalian tissue culture cells for imaging by scanning electron microscopy. Hypotonic treatment is followed by low-speed centrifugation onto polylysine-coated silicon chips, withou...
متن کاملInteraction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport.
Bidirectional movement of proteins and RNAs across the nuclear envelope requires Ran, a Ras-like GTPase. A genetic screen of the yeast Saccharomyces cerevisiae was performed to isolate conditional alleles of GSP1, a gene that encodes a homolog of Ran. Two temperature-sensitive alleles, gsp1-1 and gsp1-2, were isolated. The mutations in these two alleles map to regions that are structurally cons...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.36.22857