Radioimmunoassay of Thyroxine by T4-RIA PAC
نویسندگان
چکیده
منابع مشابه
Sensitive radioimmunoassay of total thyroxine (T4) in horses using a simple extraction method
Most thyroid hormone determinations in animals are based on immunoassays adapted from those used to test human samples, which may not reflect the actual values of thyroid hormone in horses because of the presence of binding proteins. The aims of the present study were i) to establish a novel radioimmunoassay (RIA) using a more simple and convenient method to separate binding proteins for the me...
متن کاملDetermination of free thyroxine in serum by radioimmunoassay.
We describe a sensitive and specific radioimmunoassay for free thyroxine, directly determined in the diffusate obtained from equilibrium dialysis of serum. The intra- and interassay precisions were 7.5 +/- 0.27 and 9.1 +/- 0.58 ng/liter (mean +/- SD), respectively. The free thyroxine concentrations of normal persons and patients with thyroid disease were as follows: normal, 7.6 +/- 1.7; euthyro...
متن کاملRadioimmunoassay for human thyroxine-binding prealbumin.
A radioimmunoassay (RIA) for human thyroxine-binding prealbumin (PA) is described. It employs highly purified PA, anti-human PA serum at 1:30,000 final dilution, normal bovine serum as a carrier, and polyethyleneglycol to precipitate the immune complexes. This assay is extremely sensitive (limit of detection less than 0.2 micrograms per dL or less than 3.6 X 10(-15) moles per tube), accurate (r...
متن کاملLow serum thyroxine due to anti-thyroxine antibody.
In one case of untreated Hashimoto's thyroiditis, the serum thyroxine (T4) value, obtained by radioimmunoassay (RIA) employing resin to separate bound and free T4, was significantly lower than that obtained by competitive protein binding analysis (CPBA). The discrepancy was found to be due to the presence of anti-thyroxine autoantibody in the serum. This phenomenon is considered to be of practi...
متن کاملComparison of plasma progesterone assay in women by competitive protein binding (CPB) and radioimmunoassay (RIA).
Plasma progesterone levels during the follicular and luteal phases were compared when measured simultaneously by competitive protein binding (using guinea pig sera as binding agent) and radioimmunoassay (antiserum against progesterone-11-alpha-succinyl bovine serum albumin in rabbits), the values obtained were significantly different within each technique, depending on whether previous thin-lay...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1977
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.26.8_569