Radioimmunoassay of human plasma apolipoprotein-B
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay studies of human apolipoprotein E.
This report describes the development and first applications of a sensitive and specific double antibody radioimmunoassay for human apoplipoprotein E (apoE). ApoE was purified from the very low density lipoproteins of hypertriglyceridemic patients by heparin-agarose affinity chromatography, DEAE-cellulose chromatography, and preparative polyacrylamide gel electrophoresis. The purified apoprotei...
متن کاملEffect of blood collection and processing on radioimmunoassay results for apolipoprotein B in plasma.
We studied the effects of different blood collection and processing procedures on quantification of apolipoprotein (apo) B by radioimmunoassay. High-density lipoprotein subfractions HDL3 and HDL2 and isolated apoA-I did not cross-react in the assay. Analytical recovery of apoB at different doses of very-low- and low-density lipoproteins were complete. Inter- and intra-assay coefficients of vari...
متن کاملRadioimmunoassay of Human Plasma Thyrotropin.
Many pituitary hormones possess partial or complete immunological species specificity. Earlier reports suggested that thyrotropin (TSH) did not have such specificity since the biological activity of human pituitary or plasma TSH could be neutralized by antisera to bovine TSH in doses similar to that which blocked the biological activity of bovine TSH (2, 3). However, recent studies have shown t...
متن کاملImmunochemical heterogeneity of human plasma apolipoprotein B. II. Expression of apolipoprotein B epitopes on native lipoproteins.
Eleven hybridoma antibodies specific for apoprotein B were used to examine the expression of apoprotein B epitopes on native plasma lipoproteins using fluid phase radioimmunoassays. Heterogeneity of apoprotein B in low density lipoprotein (LDL) of density 1.019-1.063 g/ml was indicated by maximum binding of radioiodinated LDL in the presence of excess antibody that ranged from 45 to 100%. Affin...
متن کاملImmunochemical heterogeneity of human plasma apolipoprotein B. I. Apolipoprotein B binding of mouse hybridoma antibodies.
Apolipoprotein B obtained from each of the major density classes of human plasma lipoproteins was investigated immunochemically. Eleven different mouse hybridoma cell lines were generated and selected on the basis of their ability to secrete antibodies that were bound by either very low density or intermediate density lipoproteins. Two of these antibodies appeared to be specific for complex epi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1976
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.4.3_209