Radioimmunoassay of Amitriptyline and Nortriptyline in Body Fluids
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of dibenzazepines and dibenzcycloheptanodienes in body fluids and tissues.
In an attempt to establish a radioimmunoassay (RIA), imipramine and amitriptyline immunogens were prepared; desmethyl derivatives were converted into hemisuccinates, conjugated with bovine serum albumin and used for rabbit immunization. [3H]Amitriptyline (4.3 TBq/mmol) and [3H]imipramine (2.9 TBq/mmol) were prepared by catalytic dehalogenation or reductive alkylation. Dibenzazepines and dibenzc...
متن کاملElectrochemical Determination of Amitriptyline using a Nanocomposite Carbon Paste Electrode in Human Body Fluids
A carbon nanocomposite electrode has been designed by incorporation of montmorillonite nanoclayinto the carbon ionic liquid electrode for the electrochemical determination of amitriptiline . The surface ofthe proposed electrode was characterized by scanning electron microscopy. The presence of ionic liquid asa binder is believed to be responsible for the electrocatalytic activity of the propose...
متن کاملQuantitative homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine.
We describe specific EMIT homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine in patients' serum samples. Before analysis, an easily performed extraction step involving the use of 500 microL of sample and a 1-mL disposable column eliminates cross-reacting polar metabolites. The range of the standard curve for the first three drugs is 25 to 250 microgram...
متن کاملA radioimmunoassay for nortriptyline (and other tricyclic antidepressants) in plasma.
The radioimmunoassay for nortriptyline described here can detect as little 1 microgram/liter of plasma. Within-day precision and day-to-day precision (CV) were +/- 6 and +/- 11%, respectively, over the concentration range 100-200 microgram/liter. The major metabolite hydroxy-nortriptyline, does not cross react with the antiserum. Results so obtained correlate closely with results by a double-is...
متن کاملMeasurement of plasma nortriptyline concentrations: radioimmunoassay and gas-chromatography compared.
We compared measurement of plasma nortriptyline by a recently developed radioimmunoassay technique with values obtained by traditional gas chromatography. The coefficient of correlation (r) was 0.98 for 84 samples from 21 controlled patients; for a separate series of 45 samples from 34 patients who were receiving in addition other medication, r was 0.96. Use of radioimmunoassay in the routine d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1978
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456327801500147