منابع مشابه
Quinoprotein alcohol dehydrogenase from ethanol-grown Pseudomonas aeruginosa.
Cell-free extracts of Pseudomonas aeruginosa strains, grown on ethanol, showed dye-linked alcohol dehydrogenase activities. The enzyme responsible for this activity was purified to homogeneity. It appeared to contain two molecules of pyrroloquinoline quinone per enzyme molecule. In many respects, it resembled other quinoprotein alcohol dehydrogenases (EC 1.1.99.8), having a substrate specificit...
متن کاملThe absence of quinoprotein alcohol dehydrogenase in Acinetobacter calcoaceticus.
It is shown that the unusual NAD(P)+-independent quinoprotein alcohol dehydrogenase, said previously to be responsible for oxidation of ethanol during growth of Acinetobacter calcoaceticus LMD 79.39, was in fact isolated from an unidentified organism which contained cytochrome c and which has now been lost. Several genuine strains of A. calcoaceticus do not contain cytochrome c nor do they cont...
متن کاملThe structure of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium extorquens.
The 1.94 A structure of methanol dehydrogenase has been used to provide a model structure for part of a membrane quinohaemoprotein alcohol dehydrogenase. The basic superbarrel structure and the active-site region are retained, indicating essentially similar mechanisms of action, but there are considerable differences in the external loops, particularly those involved in formation of the shallow...
متن کاملInvolvement of a Quinoprotein Alcohol Dehydrogenase and an NAD-dependent Aldehyde Dehydrogenase in 2-Chloroethanol Metabolism in Xanthobacter autotrophicus GJlO
An inducible methanol dehydrogenase showing high activity with 2-chloroethanol was purified from 2-chloroethanol-grown cells of the 1,2-dichloroethane utilizing bacterium Xanthobacter autotrophicus GJ10. The enzyme consisted of a 60 kDa polypeptide that was associated with a 10 kDa polypeptide and contained pyrrolo-quinoline quinone (PQQ) as a prosthetic group. Chloroethanol-grown cells of stra...
متن کاملCrystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces co...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1993
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.31.224