QuickSwitch ™: an MHC tetramer platform for making fast and reliable custom tetramers for T cell staining

Abstract The use of MHC tetramers to identify antigen specific T cells has become widespread among immunologists since the late 90s. Tetramers have so ubiquitous that many companies and research facilities can ship deliver some most commonly used within a matter days. But when it comes uncommon or with antigenic peptides only recently been identified, may be quite challenge find needed tetramer. In this case, researchers are left trying produce their own tetramer having another facility do for them, which lengthy process. an effort help those researchers, we developed QuickSwitch ™MHC This loaded place-holder peptide (or exiting peptide) exchanged well-binding given haplotype. way, able make any they available in hours. More importantly, our platform means monitor how much quantify occupancy. allows user not determine if question viable tetramer, but also strong weak binding piece information invaluable future studies, particular vaccine development. MBL International