Quantitative, Targeted Analysis of Gut Microbiota Derived Metabolites Provides Novel Biomarkers of Early Diabetic Kidney Disease in Type 2 Diabetes Mellitus Patients
نویسندگان
چکیده
Diabetic kidney disease (DKD) is one of the most debilitating complications type 2 diabetes mellitus (T2DM), as it progresses silently to end-stage renal (ESRD). The discovery novel biomarkers early DKD becomes acute, its incidence reaching catastrophic proportions. Our study aimed quantify previously identified metabolites from serum and urine through untargeted ultra-high-performance liquid chromatography coupled with electrospray ionization-quadrupole-time flight-mass spectrometry (UHPLC-QTOF-ESI+-MS) techniques, such following: arginine, dimethylarginine, hippuric acid, indoxyl sulfate, p-cresyl L-acetylcarnitine, butenoylcarnitine sorbitol. concept was based on targeted analysis selected metabolites, using 20 healthy subjects 90 T2DM patients in different stages (normoalbuminuria—uACR < 30 mg/g; microalbuminuria—uACR 30–300 macroalbuminuria—uACR > 300 mg/g). quantitative evaluation performed pure standards, followed by validation methods limit detection (LOD) quantification (LOQ). following this resulted possible DKD: serum—arginine, sorbitol urine—p-cresyl sulfate.
منابع مشابه
Gut microbiota and type 2 diabetes mellitus.
In recent years, many studies have related gut microbiome to development of highly prevalent diseases such as type 2 diabetes and obesity. Obesity itself is associated to changes in the composition of gut microbiome, with a trend to an overgrowth of microorganisms more efficiently obtaining energy from diet. There are several mechanisms that relate microbiota to the onset of insulin resistance ...
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چکیده ندارد.
15 صفحه اولEndothelin-1 gene polymorphisms and diabetic kidney disease in patients with type 2 diabetes mellitus.
BACKGROUND AND AIMS Diabetic kidney disease (DKD) is the leading cause of end stage renal disease worldwide and is associated with increased cardiovascular mortality. The endothelin system has been implicated in the pathogenesis of arterial hypertension and renal dysfunction. In the present study, the association of DKD with polymorphisms in ET-1 (EDN1) and ETRA (EDNRA) genes was analyzed in pa...
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ژورنال
عنوان ژورنال: Biomolecules
سال: 2023
ISSN: ['2218-273X']
DOI: https://doi.org/10.3390/biom13071086