Quantitative Paper Chromatography. II

The quantitative separation of estrogens by paper partition chromatography.

The varying success of known chromat’ographic procedures for the separation and identification of microquantities of estrogens (1, 2) has led to the development of paper partit!ion chromatographic techniques which are reported here. These t’echniques permit rapid separation, identification, and quantitative estimation of free est.rogens in biological materials. The solvent systems of o-dichloro...

Quantitative paper chromatography of conjugated 17-ketosteroids in plasma.

The determination of urinary 17-ketosteroids has limited applications in the understanding and diagnosis of the nature of endocrine disorders associated with mild hirsutism and virilism in the female, because a very large number of these patients excretes 17-ketosteroids well within the normal range. Recent experience of Finkelstein, Forchielli and Dorfman (1) suggests that in these cases the b...

Quantitative paper chromatography of reducing steroids of the adrenal cortex.

T. MANN AND E. LEONE I953 Neuberger, A. & Webster, T. A. (1946). Biochem. J. 40, 576. Newton, E. B., Benedict, S. R. & Dakin, H. D. (1926). Science, 64, 602. Pauly, H. (1904). Hoppe-Seyl. Z. 42, 508. Pirie, N. WV. (1933a). Biochem. J. 27, 202. Pirie, N. W. (1933 b). Biochem. J. 27, 1181. Potter, Van R. & Franke, K. W. (1935). J. Nutr. 9, 1. Roe, J. H. & Kuether, C. A. (1943). J. biol. Chem. 147...

Determination of monoamino monocarboxylic acids by quantitative paper chromatography.

Many methods for chromatographic separation of amino acids are now available, but their subsequent determination, generally employing ninhydrin, often leaves much to be desired. It was felt, therefore, that there was a need for a simple method of analysis based on the convenient paper technique and employing some means of determination less fickle than ninhydrin colorimetry. The method now pres...

Study of phenylketonuria by paper chromatography