Quantitative monitoring of galactolipid hydrolysis by pancreatic lipase-related protein 2 using thin layer chromatography and thymol-sulfuric acid derivatization
نویسندگان
چکیده
Galactolipids are the most abundant lipids on earth where they mainly found in photosynthetic membranes of plant, algae, and cyanobacteria. Pancreatic lipase-related protein 2 (PLRP2) is an enzyme with galactolipase activity allowing mammals, especially herbivores, to digest this important source fatty acids. We present a method for quantitative analysis galactolipids galactosylated products resulting from their digestion by guinea pig PLRP2 (GPLRP2), using thin-layer-chromatography (TLC), thymol-sulfuric acid as derivatization reagent scanning densitometry detection. Thymol-sulfuric has been used colorimetric detection carbohydrates. It shown here that galactosyl group not affected bound acyl glycerol, chains length number galactose residues polar head. This allowed quantifying simultaneously initial substrate all generated upon hydrolysis monogalactosyl di-octanoylglycerol (C8-MGDG) GPLRP2 single calibration C8-MGDG reference standard. The reaction products, monooctanoyl glycerol (C8-MGMG) (MGG), were identified quantified, MGG being recovered aqueous phase analyzed separate TLC analysis. therefore suitable quantify release both acids MGDG thereby shows can contribute complete further intestinal absorption
منابع مشابه
Identification of amino acid thiohydantoins directly by thin-layer chromatography and indirectly by gas-liquid chromatography after hydrolysis.
A method is described for the identification of amino acid thiohydantoins by two-dimensional t.l.c. An indirect method for the determination of amino acid thiohydantoins is described which, after hydrolysis, the corresponding amino acids are determined by g.l.c.
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UNLABELLED An accurate, simple and low-cost image-analysis system has been set up for capturing thin-layer chromatography images, and a software package, TLCQA, has also been developed for both qualitative and quantitative analyses using the acquired image. AVAILABILITY Available upon request from the authors. CONTACT [email protected]
متن کاملChristhin: Quantitative Analysis of Thin Layer Chromatography
A copy of the license is included in the section entitled " GNU Free Documentation License ". Acknowledgments We thank CONICET, the Secretaría de Ciencia, Tecnologia e Innovación, Gobierno de la Provincia de Chubut and the Consejo Interuniversitario Nacional (CIN) for supporting this development. We also thank Dr. Héctor " Tito " Fernández and Dr. Nazareno Aguirre for their help.
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ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2021
ISSN: ['1570-0232', '1873-376X']
DOI: https://doi.org/10.1016/j.jchromb.2021.122674