Quantitative gas-liquid chromatography of fatty acid methyl esters with the thermal conductivity detector
نویسندگان
چکیده
منابع مشابه
Preparation of fatty acid methyl esters for gas-liquid chromatography.
A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol....
متن کاملPreparation of bacterial Fatty Acid methyl esters for rapid characterization by gas-liquid chromatography.
Our recent survey (B. L. Brian and E. W. Gardner, unpublished data) of fatty acids extracted from a variety of bacterial species (Escherichia coli, Staphylococcus aureus, Sarcina lutea, Serratia marcescens, Salmonella shottmuelleri, Proteus vulgaris, Bacillus subtilis, Pseudomonas fluorescens, and several strains of Vibrio cholerae) called for a rapid, reproducible technique for extraction, est...
متن کاملComputer treatment of gas-liquid chromatographic data, with special reference to fatty acid methyl esters.
A computer program is described which analyzes output punched directly onto paper tape from a gas-liquid chromatograph. Although this program was written specifically for samples of fatty acid methyl esters derived from adipose tissue triglycerides which are eluted within 1 hr, modification of the dimension statements in the program would enable it to deal with samples which require a longer ti...
متن کاملCharacterization of benthic microbial community structure by high-resolution gas chromatography of Fatty Acid methyl esters.
Fatty acids are a widely studied group of lipids of sufficient taxonomic diversity to be useful in defining microbial community structure. The extraordinary resolution of glass capillary gas-liquid chromatography can be utilized to separate and tentatively identify large numbers of fatty acid methyl esters derived from the lipids of estuarine detritus and marine benthic microbiota without the b...
متن کاملAnalysis of fatty acid methyl esters by a gas--liquid chromatography--chemical ionization mass spectrometry computer system.
The technique of gas-liquid chromatography-chemical ionization mass spectrometry can easily identify trace peaks and unresolved peaks on gas-liquid chromatography, utilizing MH(+) ions of chemical ionization mass spectra. In polyunsaturated fatty acid methyl esters such as C22:5 and C22:6, the determination of molecular weights that are difficult to determine by electron impact mass spectrometr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1961
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39046-5