Quantitative Determination of Neutral Lipids on Thin Layer-FID Chromatography
نویسندگان
چکیده
منابع مشابه
Thin-layer Chromatography of Lipids
There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...
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THIN-LAYER CHROMATOGRAPHY (TLC) has been widely applied to the qualitative analysis of complex biologic lipid mixtures (1, 2), as well as to the quantitation of selected biochemical entities (3). Quantitation by TLC has been limited,for the most part,to the recovery of separated components from the chromatoplate, followed by an independent analysis using established analytic methods. Current te...
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UNLABELLED An accurate, simple and low-cost image-analysis system has been set up for capturing thin-layer chromatography images, and a software package, TLCQA, has also been developed for both qualitative and quantitative analyses using the acquired image. AVAILABILITY Available upon request from the authors. CONTACT [email protected]
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A copy of the license is included in the section entitled " GNU Free Documentation License ". Acknowledgments We thank CONICET, the Secretaría de Ciencia, Tecnologia e Innovación, Gobierno de la Provincia de Chubut and the Consejo Interuniversitario Nacional (CIN) for supporting this development. We also thank Dr. Héctor " Tito " Fernández and Dr. Nazareno Aguirre for their help.
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The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...
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ژورنال
عنوان ژورنال: Journal of Japan Oil Chemists' Society
سال: 1976
ISSN: 1884-2003
DOI: 10.5650/jos1956.25.263