Quantitative assay of enzymes in tissues.

Quantitative assay of enzymes in tissues.

The quantitative significance of the transsulfuration enzymes for H2S production in murine tissues.

The enzymes of the transsulfuration pathway, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), are important for the endogenous production of hydrogen sulfide (H(2)S), a gaseous signaling molecule. The relative contributions of CBS and CSE to H(2)S generation in different tissues are not known. In this study, we report quantification of CBS and CSE in murine liver and kidney and t...

Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed.

β-glucuronidase (GUS) is a useful reporter for the evaluation of promoter characteristics in transgenic plants. Here, we introduce an original technique to quantify the strength of promoters at subtissue resolution of cell clusters. The method combines cryotomy, laser microdissection, and improved fluorometric analysis of GUS activity using 6-chloro-4-methylumbelliferyl-β-D-glucuronide as an ef...

The assay of animal tissues for respiratory enzymes; the malic dehydrogenas system.

The “homogenate technique” is at present widely used for the study of enzymatic reactions in various types of biological material. In the original technique (1)’ an attempt n-as made to eliminat,e cell structure and to disperse intracellular constitucnt,s so widely that endogenous respiration was eliminated by dilution, while particular phases of metabolism could be restored by the addition of ...

Quantitative assay and subcellular distribution of enzymes acting on dolichyl phosphate in rat liver

To establish on a quantitative basis the subcellular distribution of the enzymes that glycosylate dolichyl phosphate in rat liver, preliminary kinetic studies on the transfer of mannose, glucose, and N-acetylglucosamine-1-phosphate from the respective (14)C- labeled nucleotide sugars to exogenous dolichyl phosphate were conducted in liver microsomes. Mannosyltransferase, glucosyltransferase, an...