Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

Abstract In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. work we aimed to shed light on factors involved in successful vitro development haploid by evaluating role growth regulators (GRs) process. We analyzed endogenous levels different GRs, including auxins, cytokinins gibberelins, well salicylic, jasmonic abscisic acid, microspores microspore-derived at culture stages. also same GR profiles leaf anther wall tissues backgrounds. Finally, assessed application combinations medium. Our results showed there no genotype-specific can be associated a high embryogenic response. Instead, response seems related accumulation patterns during culture. The changes observed salicylic acid were not embryo transition. There were, however, indole acetic dihydrozeatin. best combination promote callus production was 0.5 mg/L 1-naphthaleneacetic (NAA) 6-benzylaminopurine (BAP). A 20% reduction NAA BAP reduced but structures more anatomically similar These GRs cultures.