Quantitative Analysis Using Raman Spectrometry

Quantitative metabolome analysis using capillary electrophoresis mass spectrometry.

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstr...

Ultraviolet Raman Spectrometry

Non-invasive analysis in micro-reactors using Raman spectrometry with a specially designed probe.

An optical interface has been designed to maximise the sensitivity and spatial resolution required when Raman spectrometry is used to monitor a reaction in a micro-reactor, revealing advantages over a conventional commercial probe. A miniature aspheric lens was shown to be better than microscope objectives to focus the probing laser beam onto the sample. The diameters of the exciting and collec...

Studies of particle drying using non-invasive Raman spectrometry and particle size analysis.

The evaporation of methanol from needle-shaped particles of cellobiose octaacetate (COA) has been studied directly in a jacketed vacuum drier using in situ measurements by Raman spectrometry. A design of experiments (DoE) approach was used to investigate the effects of three parameters (method of agitation, % solvent loss on drying and jacket temperature), with the intention of minimising the d...

Quantitative enantioselective Raman spectroscopy.

Analytical methods for quantitative enantioselective measurements are highly desirable in the life sciences. Existing technologies have disadvantages such as limited temporal resolution, the need for molecular labeling, or high experimental complexity. To overcome these limitations, this work presents a method based on conventional Raman spectroscopy. A systematic investigation of the key param...