Quantitative analysis of sperm-egg interaction in the zona-free hamster egg penetration test
نویسندگان
چکیده
منابع مشابه
Relationship between human in-vitro fertilization and intracytoplasmic sperm injection and the zona-free hamster egg penetration test.
The zona-free hamster egg penetration test (HEPT) is widely used for evaluating the fertilizing ability of human spermatozoa. However, the relationship between the HEPT and microassisted fertilization has yet to be determined. To evaluate the efficiency of HEPT in selecting the most appropriate method of in-vitro fertilization (IVF), including intracytoplasmic sperm injection (ICSI) in couples ...
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In most mammals that have been studied, the first spermatozoon which penetrates an egg stimulates the egg to undergo a reaction—the 'zona reaction'—which bars further spermatozoa from penetrating through the zona pellucida (Braden, Austin & David, 1954). The sequel to the zona reaction may be classified into two categories, according to species: species in which spermatozoa are entirely prevent...
متن کاملThe predictive value of the zona-free hamster egg penetration test in relation to in-vitro fertilization at various insemination concentrations.
The aim of the study was to evaluate the predictive value of the zona-free hamster egg penetration test (ZHEPT) for success in in-vitro fertilization (IVF) at various insemination concentrations ranging between 0.1 and >0.6 x 10(6)/ml. The ZHEPT was assessed using sperm samples from 87 couples undergoing IVF treatment. A similar test was simultaneously performed on the same semen sample followi...
متن کاملZona-free hamster ovum penetration by human spermatozoa: influence of various sperm factors.
The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemina...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1986
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1986.tb00940.x