Quantitative analysis of CD34+ stem cells using RT-PCR on whole cells.
نویسندگان
چکیده
منابع مشابه
Expansion of Non-Enriched Cord Blood Stem/Progenitor Cells CD34+ CD38- Using Liver Cells
Many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. The proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. Using primary fetal liver cells, we established...
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متن کاملexpansion of non-enriched cord blood stem/progenitor cells cd34+ cd38- using liver cells
many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. the proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. using primary fetal liver cells, we established...
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One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...
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bromide staining in detecting PCR products (Figure 3). Moreover, as expected, band intensities increase with longer duration of exposure to X-ray film (data not shown). Biotin-21-dUTP and biotin-14-dATP work equally well in labeling the PCR products (data not shown). Similarly, DNA cross-linking with baking and UV irradiation work equally well in this system (data not shown). We have also confi...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1994
ISSN: 1088-9051
DOI: 10.1101/gr.3.5.278