Quantification of major royal jelly proteins using ultra performance liquid chromatography tandem triple quadrupole mass spectrometry and application in honey authenticity

Major royal jelly proteins (MRJPs) have been used as endogenous biomarkers for honey authentication. However, no study on quantification of MRJPs in authentication was reported. In order to develop a new authenticity method based quantitative analysis honey, trypsin-digested peptides from were examined by mass spectrometry (MS). Three peptides, YNGVPSSLNVISK, TLQMIAGMK, and LTVAGESFTVK selected specific markers MRJP1, MRJP 2 3(MRJP1?3), respectively. An ultra performance liquid chromatography-triple quadrupole MS (UPLC-TQMS) developed validated. A database the MRJP1?3 contents established 70 authentic samples showed potential application identification. The results 10 commercial UPLC-TQMS with reference compared confirmed widely isotope ratio spectrometry, AOAC Official Method. conclusion, our method, proteomic-based has high accuracy, sensitivity specificity authenticity.