Quantification of chemotherapeutic target gene mRNA expression in human breast cancer biopsies: Comparison of real-time reverse transcription-PCR vs. Relative quantification reverse transcription-PCR utilizing DNA sequencer analysis of PCR products
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چکیده
منابع مشابه
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can combine high sensitivity with reliable specificity. The technology is evolving rapidly with the introduction of new en...
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BACKGROUND Competitive PCR of reverse transcribed mRNA sequences is used to quantify transcripts, but the usual approaches are labor-intensive and time-consuming. We describe the non-gel-based quantification of competitive reverse transcription (RT)-PCR products with use of microparticles and flow cytometry. METHODS PCR products of a target sequence and an internal control sequence (competito...
متن کاملReal-Time Reverse Transcription PCR
Real-time, fluorescence-based reverse transcription polymerase chain reaction (RT-PCR) has been transformed from an experimental technology into a mainstream scientific tool for the detection of RNA. This is because of several factors: 1) it is a homogeneous assay, which eliminates the requirement for post-PCR processing; 2) it has a wide dynamic range; 3) there is little interassay variation; ...
متن کاملRapid quantification of CYP3A4 expression in human leukocytes by real-time reverse transcription-PCR.
The SD of residuals (S y͉x) was 73 ng/L in the concentration range of 0 –5000 ng/L and 22 ng/L in the range of 0 –500 ng/L. The agreement between the GC/MS and MEIA assays indicated low interference from other estrogenic compounds and their metabolites in the latter assay. In conclusion, we have developed a GC/MS method for the quantification of estradiol in patient serum samples and serum-based...
متن کاملCritical analysis of rhinovirus RNA load quantification by real-time reverse transcription-PCR.
Rhinoviruses are the most frequent cause of human respiratory infections, and quantitative rhinovirus diagnostic tools are needed for clinical investigations. Although results obtained by real-time reverse-transcription PCR (RT-PCR) assays are frequently converted to viral RNA loads, this presents several limitations regarding accurate virus RNA quantification, particularly given the need to re...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2003
ISSN: 0887-8013,1098-2825
DOI: 10.1002/jcla.10091