Quantification and varietal variation of low molecular weight glutenin subunits (LMW-GS) using size exclusion chromatography
نویسندگان
چکیده
منابع مشابه
variation of the high molecular weight glutenin subunit (hmw-gs) in iranian accessions of aegilpos crassa
one hundred and twenty iranian accessions of aegilops crassa with origin from different geographical areas of iran, were evaluated for variation in high molecular weight glutenin subunit (hmw-gs) composition. the results showed different banding patterns between hexa (6x) and tetra-ploid (4x) as well as within different ploidy accessions. eleven allelic variants were observed in glu- dcr1 locus...
متن کاملAllelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars
Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the G...
متن کاملارزش آزمون رسوب در برآورد کیفیت نانوایی گندم و توجیه تغییرات آن از راه تجزیه زیرواحدهای گلوتنین با روشRP-HPLC
In order to evaluate the relationship between SDS-sedimentation value and breadmaking quality of wheat (Triticum aestivum L.), glutenin subunits of different genotypes (foreign and Iranian cultivars) were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). SDS-sedimentation value was used as an indirect criterion for breadmaking quality. Correlation coefficients revealed...
متن کاملارزش آزمون رسوب در برآورد کیفیت نانوایی گندم و توجیه تغییرات آن از راه تجزیه زیرواحدهای گلوتنین با روشRP-HPLC
In order to evaluate the relationship between SDS-sedimentation value and breadmaking quality of wheat (Triticum aestivum L.), glutenin subunits of different genotypes (foreign and Iranian cultivars) were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). SDS-sedimentation value was used as an indirect criterion for breadmaking quality. Correlation coefficients revealed...
متن کاملIdentification of Intact High Molecular Weight Glutenin Subunits from the Wheat Proteome Using Combined Liquid Chromatography-Electrospray Ionization Mass Spectrometry
The present paper describes a method for the identification of intact high molecular weight glutenin subunits (HMW-GS), the quality determining proteins from the wheat storage proteome. The method includes isolation of HMW-GS from wheat flour, further separation of HMW-GS by reversed-phase high-performance liquid chromatography (RP-HPLC), and their subsequent molecular identification with elect...
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ژورنال
عنوان ژورنال: Journal of Food Science and Technology
سال: 2018
ISSN: 0022-1155,0975-8402
DOI: 10.1007/s13197-017-2995-8