QTL mapping and analysis of candidate genes in flag leaf morphology in rice

Leaf is an important organ of rice, and it the main place for photosynthesis. Leaf morphology related traits such as leaf length, width area can directly affect photosynthetic efficiency. The study quantitative trait loci (QTL) sites morphological characters rice flag improvement by means genetic breeding provide theoretical basis cultivation ideal plant type rice. In this study, indica variety HZ was used male parent, a japonica Nekken2 female parent. After F1 generation obtained cross-breeding, 120 recombinant inbred lines (RIL populations) were from successive generations self-crossing, which experimental materials in study. width, at tillering stage mature studied analyzed after incubation under same appropriate conditions. At time, map constructed using RIL population to locate QTLs stage, 35 detected. There 6 blade length (5 1 maturation stage), LOD value one them high 6.92; 8 (detected only 4.51; 21 (12 9 values two 6.37 4.06 respectively. Through analysis candidate genes these QTL regions, found that LOC_Os02g52040, LOC_Os03g46610, LOC_Os04g52230, LOC_Os04g42230, LOC_Os04g44150, LOC_Os04g52479, LOC_Os09g37400 and LOC_Os12g42020 significantly different expression levels between parents. Combined with data parents, we speculated that expressions of LOC_Os04g52230, LOC_Os04g44150, may promote growth area, while LOC_Os02g52040, LOC_Os03g46610 LOC_Os04g52479 may inhibit leaves. regulating excavated, provided reference cultivating new varieties excellent type.