Purification of RNA-dependent RNA polymerase from brome mosaic virus-infected barley leaves.
نویسندگان
چکیده
منابع مشابه
Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.
The association of host proteins with viral RNA replication proteins has been reported for a number of (+)-strand RNA viruses. However, little is known about the identity or function of these host proteins in viral replication. In this paper we report the characterization of a host protein associated with the RNA-dependent RNA polymerase (RdRp) from brome mosaic virus (BMV)-infected barley. A h...
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Previously, we have observed that mutations in proteins 1a and 2a, the two virally encoded components of the brome mosaic virus (BMV) replicase, can affect the frequency of recombination and the locations of RNA recombination sites (P. D. Nagy, A. Dzianott, P. Ahlquist, and J. J. Bujarski, J. Virol. 69:2547-2556, 1995; M. Figlerowicz, P. D. Nagy, and J. J. Bujarski, Proc. Natl. Acad. Sci. USA 9...
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Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
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The rates of virus RNA synthesis and virion accumulation were investigated in brome mosaic virus-infected barley protoplasts. Single-stranded virus RNAs could be detected as early as 6 h after inoculation. Only RNA components I and z were detected at this time, suggesting that their synthesis is initiated relatively early in infection. The RNAs were synthesized at similar rates from t 6 to 35 h...
متن کاملEnhancer-like activity of a brome mosaic virus RNA promoter.
As with transcription from DNA templates, RNA synthesis from viral RNA templates must initiate accurately. RNA sequences named specificity and initiation determinants allow recognition of and coordinated interaction with the viral replication enzyme. Using enriched replicase from brome mosaic virus (BMV)-infected plants and variants of the promoter template for minus-strand and subgenomic RNA i...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1984
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.50.491