Purification of Recombinant Eel Growth Hormone.
نویسندگان
چکیده
منابع مشابه
Large-scale purification and refolding of recombinant eel growth hormone.
In the past decade, growth hormones (GHs) have been isolated from the pituitaries of several teleost species including tilapia, salmon, carp, and eel.1~9) Although teleost GHs may be useful in promoting growth in aquaculture, little is known about appropriate routes, doses, or patterns of administration. Considerable amounts of teleost GHsare required to clarify both the mode of action of teleo...
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Production and purification of human growth hormone using a simple method was studied in two recombinantEscherichia coli, D7-5 and C27-2 strains. The r-hGH was expressed in the form of inclusion body in a batchfermentation process and purified to 99% purity using a procedure based on acid precipitation of the hostderived proteins and other impurities. The effect of the pH and ...
متن کاملPersian sturgeon growth hormone elaboration and purification
In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...
متن کاملPersian sturgeon growth hormone elaboration and purification
In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...
متن کاملPurification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli.
The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively replaces in the maternal bloodstream. hGH-V cDNA has previously been cloned and isolated. Analysis of its nucleotide sequence has revealed a 191-residue protein, hPGH, differing from hGH at 13 p...
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ژورنال
عنوان ژورنال: KAGAKU KOGAKU RONBUNSHU
سال: 1991
ISSN: 0386-216X,1349-9203
DOI: 10.1252/kakoronbunshu.17.620