Purification of Japanese encephalitis virus vaccine by zonal centrifugation

Purification of bacterial endotoxins by zonal centrifugation.

In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen....

Purification of DNA-origami nanostructures by rate-zonal centrifugation

Most previously reported methods for purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation. Although AGE is routinely used to yield 0.1-1 µg purified DNA nanostructures, obtaining >100 µg of purified DNA-origami structure through AGE is typically laborious because of the post-electrophoresis extraction, desalting and concentration steps. Here, we present ...

Large-scale purification of Japanese encephalitis virus from infected mouse brain for preparation of vaccine.

Large volumes of Japanese encephalitis (JE) virus propagated in mouse brain can be easily purified by polyethylene glycol 6,000. By using the polyethylene glycol precipitation method, mouse hemoglobin was almost all separated from the viral suspension, and consequently the total amount of nonviral protein in the viral suspension decreased. The recovery of infectivity was about 100%. The removal...

Japanese Encephalitis Vaccine

clinic in preparation for a trip to Asia. They are then faced with the seemingly impossible decision of whether to spend money on a vaccine that has a rather bad reputation or to risk developing the disease, which would be much worse.1 Many of the physicians that have to advise them feel ill equipped to do so. From a Western perspective, JE is considered a rare and exotic disease, but for those...

Purification of Tomato Bushy Stunt Virus by Differential Centrifugation