Purification of Inorganic Substances

Purification of yeast inorganic pyrophosphatase.

An enzyme which hydrolyzes inorganic pyrophosphate (PP) to orthophosphate (ortho P) was demonstrated by Lohmann (1) in muscle. Bauer (2) found that autolysates of slowly dried bottom yeast were a rich source of inorganic pyrophosphatase and were free of adenosinetriphosphatase (ATPase) activity. An important contribution was made by Bailey and Webb (3) who accomplished an extensive purification...

The Polymerisation of Formaldehyde in Presence of Inorganic Substances.

Inorganic Phosphorus in Plant Substances. an Improved Method of Estimation

In the synthesis of organic phosphorus compounds by the plant and in their storage in the seed as reserve food the inorganic phosphates play an important part. The phosphorus used in this synthetic process is taken into the plant in inorganic form and used in the building up of complex organic phosphorus compounds such as lecithins, phosphoand nucleo-prot,eins and salts of phytic acid. It is be...

Effects of expanded clay (Leca) and Styrofoam as inorganic growing media substances on growth and development of Spathiphyllum wallisii

The present study was conducted to evaluate the effect of expanded clay (Leca) and Styrofoam on the physical characteristics of growing media and their relationship with the growth of Spathiphyllum wallisii. The experiment was laid out in a complete randomized design (CRD) with 9 treatments and 3 replications. Cocopeat and peat moss combined with different proportions of inorganic materials Lec...

Purification and some properties of inorganic pyrophosphatase from human erythrocytes.

Inorganic pyrophosphatase was purified BOO-fold from human erythrocytes by a procedure involving complete hemolysis of the cells, removal of hemoglobin, ammonium sulfate fractionation, diethylaminoethyl cellulose column chromatography, and gel filtration. Magnesium chloride and 2-mercaptoethanol stabilized the activity. The procedure yielded an enzyme with an optimal pH of 7.7, and an apparent ...