Purification of His-tagged Proteins with [Desthiobiotin-BSA-EDTA] Conjugates Exhibiting Resistance to EDTA
نویسندگان
چکیده
منابع مشابه
Purification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1.
The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities we...
متن کاملEDTA Chelation Therapy
This study measured excretion of 11 minerals induced by EDTA chelation therapy in 25 adults (aluminum, cadmium, calcium, chromium, copper, iron, lead, magnesium, manganese, mercury, zinc). We administered 20 EDTA infusions (3 g, with magnesium and ascorbate) 1 to 2 weeks apart and measured 24-hr urinary excretions of minerals before treatment and after treatments 1, 10, and 20. A modest supplem...
متن کاملEDTA-dependent lymphocyte clumping.
A 79-year old man presented with chest pain and fatigue. He was found to have anemia (hemoglobin 8.8 g/dL) and leukocytosis (white blood cell count 244x10/L) with a predominance of lymphocytes (94%). Flow cytometric study and flourescence in situ hybridization analysis of the peripheral blood lymphocytes were consistent with a diagnosis of mantle cell lymphoma. A peripheral smear obtained from ...
متن کاملEDTA (Ethylenediaminetetraacetic Acid)
Ethylenediaminetetraacetic acid (EDTA) was developed by Franz Munz inGermanyduring the 1930s as an alternative to citric acid. About the same time, Frederick Bersworth synthesized EDTA in the United States using a different technique. He obtained aUSpatent for this technique in1945. EDTAwas approvedby the US Food and Drug Administration (FDA) as a food additive in 1947. Since the early 1950s, E...
متن کاملTandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins--evaluation of two anti-His-tag monoclonal antibodies.
A tag comprising four to six histidines genetically fused to the protein of interest (His-tag) has been widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). Here we report the utilization of the same tag twice in series, first for IMAC and subsequently for immunoaffinity purification. Both steps are based on completely different physical principles and can the...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2008
ISSN: 1043-1802,1520-4812
DOI: 10.1021/bc800123j