Purification of Giardia muris cysts by velocity sedimentation
نویسندگان
چکیده
منابع مشابه
Inactivation of Giardia muris cysts by free chlorine.
The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7, and 9 at 25 degrees C and pH 7 at 5 degrees C. Survival curves were "steplad...
متن کاملLow pressure ultraviolet studies for inactivation of Giardia muris cysts.
AIMS The research was initiated to confirm earlier ultraviolet (u.v.) light inactivation studies performed on Giardia cysts using excystation as the viability indicator. Following this, a comparison of in vitro excystation and animal infectivity was performed for assessing cyst viability after exposure to low-pressure u.v. irradiation. METHODS AND RESULTS Cysts of Giardia muris were inactivat...
متن کاملComparison of three different methods for concentration and purification of giardia lamblia cysts
Background: Giardia lamblia is the most frequent human intestinal protozoan worldwide. Diagnosis of G. lamblia by microscopic examination of stool is common, but ELISA & IFA methods have been used in research and epidemiological studies recently. To produce polyclonal antibodies for designing ELISA & IFA kites molecular and genomic studies (PCR and parasitic culture) as well as other epidemiolo...
متن کاملBatch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water.
AIM To determine whether batch solar disinfection (SODIS) can be used to inactivate oocysts of Cryptosporidium parvum and cysts of Giardia muris in experimentally contaminated water. METHODS AND RESULTS Suspensions of oocysts and cysts were exposed to simulated global solar irradiation of 830 W m(-2) for different exposure times at a constant temperature of 40 degrees C. Infectivity tests wer...
متن کاملMagnetic Separation of Giardia Cysts from Water
Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water. Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 c...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1984
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.48.2.454-455.1984