Purification of commercial Trypan Blue by silicic acid column chromatography
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چکیده
منابع مشابه
Microdetermination of cholesterol and phospholipid in cerebrospinal fluid and serum by silicic acid column chromatography.
A method is presented for the determination of cholesterol and phospholipid, which requires 5 I . of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 zg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 sg. of cholesterol and 30.5 g. of phospholi...
متن کاملThe fractionation of cholesterol esters by silicic acid chromatography.
Within recent years, the technique of silicic acid chromatography to separate complex mixtures of lipides has been developed widely (1-3). At the present time it is possible to resolve some eight different classes of lipides ranging from hydrocarbons to phospholipides on a single column (4). In addition, this procedure has been used to separate classes of phospholipides from one another (5). Ou...
متن کاملSeparation of Glycosyl Diglycerides from Phosphatides Using Silicic Acid Column Chromatography.
A procedure has been developed for the separation of the glycosyl diglycerides from the phosphatides of Gram-positive bacteria on columns of silicic acid. The method utilizes mixtures of acetone in chloroform for elution of the glycosyl diglycerides, followed by increasing amounts of methanol in chloroform for elution of the phosphatides. The course of the fractionation was followed by means of...
متن کاملPurification of pituitary autoantigen by column liquid chromatography and chromatofocusing
INTRODUCTION Pituitary autoantibodies can be determined both in patients with pituitary disease as well as patients with autoimmune endocrine diseases. The purpose of the study was to isolate and purify pituitary autoantigen using sera of patients and the microsomal fraction of the pituitary. MATERIAL AND METHODS To isolate a pituitary autoantigen, patient sera were used, which showed a stron...
متن کاملPurification of human plasma haptoglobin by hemoglobin-affinity column chromatography.
Haptoglobin (Hp) is an acute-phase protein; its plasma levels increase consistently in response to infection and inflammation. The concentration of human plasma Hp is ranged between 1 and 1.5 mg/ml. Similar to blood type, individual human Hp is classified as Hp 1-1, 2-1, or 2-2. The structural and functional analysis of the Hp, however, has not been studied in detail due to its difficult isolat...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1984
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.33.8_397