منابع مشابه
PARTIAL PURIFICATION AND CHARACTERIZATION OF B-GALACTOSIDASE FROM ASPERGILLUS NIGER UV-5
The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...
متن کاملPurification of cerebroside galactosidase from rat brain.
A method is described for the preparation of a B-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between I 000-13 ooo xg from rat brains and is extracted with a 60,& sodium cholate solution. The enzyme is solubilized and inactive proteins removed by digestion with pancreatic enzymes and the cholate is removed by dialysis and...
متن کاملpartial purification and characterization of b-galactosidase from aspergillus niger uv-5
the enzyme pgalactosidase from a mutant strain of a. niger uv-5 was partially purified using ammonium sulfate and acetone. the saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. the combined procedures of 1.5 v...
متن کاملPurification and characterization of the beta-galactosidase of Aeromonas formicans.
Rohlfing, S. R. (Western Reserve University, Cleveland, Ohio), and I. P. Crawford. Purification and characterization of the beta-galactosidase of Aeromonas formicans. J. Bacteriol. 91:1085-1097. 1966.-The beta-galactosidase of Aeromonas formicans was purified by diethylaminoethyl cellulose chromatography and gel filtration on Sephadex G-200. The properties of the enzyme molecule were compared w...
متن کاملThe purification and properties of beta-galactosidase from bovine testes.
A /3-galactosidase was purified 600-fold from bovine testes by ammonium sulfate precipitation, acetone fractionation, and at5nity chromatography on agarose substituted with terminal thio-fl-galactopyranosyl residues. The preparation was devoid of protease, a-fucosidase, ar-mannosidase, pglucosidase, P-glucuronidase, and hyaluronidase activities. A wide variety of compounds containing terminal n...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1969
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2061