Purification and use of E. coli peptide deformylase for peptide deprotection in chemoenzymatic peptide synthesis
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چکیده
منابع مشابه
Purification and use of E. coli peptide deformylase for peptide deprotection in chemoenzymatic peptide synthesis.
Peptide deformylases (PDFs) catalyze the removal of the formyl group from the N-terminal methionine residue in nascent polypeptide chains in prokaryotes. Its deformylation activity makes PDF an attractive candidate for the biocatalytic deprotection of formylated peptides that are used in chemoenzymatic peptide synthesis. For this application it is essential to use PDF preparations that are free...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2013
ISSN: 1046-5928
DOI: 10.1016/j.pep.2013.01.004