Purification and selected biochemical properties of peroxidase from cress (Lepidium sativum sub sp. sativum)
نویسندگان
چکیده
منابع مشابه
Enzymic Properties of Purified Myrosinase from Lepidium sativum Seedlings
To establish the substrate specificity o f the thioglucoside glucohydrolase myrosinase (EC 3.2.3.1), this enzyme was purified to homogeneity from light-grown cress (Lepidium sativum L.) seedlings by Sephadex gel filtration. Red Dye and anion exchange (FPLC M ono Q) chro matography, and preparative isoelectric focusing. Hydrolytic activity was shown toward only 4 o f the 29 synthetic and natura...
متن کاملNourishing and healing prowess of garden cress (Lepidium sativum Linn.)− A review
Garden cress has been considered as an important medicinal plant since the Vedic era. In Ayurveda, it is described as hot, bitter, galactogogue and aphrodisiac and claimed to destroy Vata (air) and Kapha (phlegm). In Unani system of medicine, seeds and leaves of this plant have been reported to possess diuretic, aperient and aphrodisiac properties and are recommended in inflammation, bronchitis...
متن کاملOn the expression of several Lhc genes in garden cress (Lepidium sativum L.).
The polymerase chain reaction was used to prepare gene-specific probes for several Lhc genes coding for chlorophyll a/b-binding proteins of cress (Lepidium sativum L.). Due to the presence of about 150 basepairs of the coding region, the isolated clones could be attributed to Lhc a3 (1 clone), Lhc b1 (5 clones), Lhc b2 (1 clone) and Lhc b3 (1 clone) genes. Probes prepared from the 3'non-coding ...
متن کاملGlucosinolate Biosynthesis: Sulfation of Desulfobenzylglucosinolate by Cell-Free Extracts of Cress (Lepidium sativum L.) Seedlings.
After removal of myrosinase activity by concanavalin A-Sepharose 4B chromatography, cell-free extracts of light-grown cress (Lepidium sativum L.) seedlings, catalyzed the sulfation of desulfobenzylglucosinolate (K(m), 0.23 millimolar) to benzylglucosinolate using PAPS (K(m), 1 millimolar) as sulfur donor. Sulfotransferase activity, which was optimal at pH 9.0, was stimulated by MgCl(2), MnCl(2)...
متن کاملEffect of citric acid on antioxidant activity of garden cress (Lepidium sativum L.) under chromium stress
Chromium-VI would impose detrimental effects morphological, physiological, and metabolic in plants. This study aimed to research the impact of citric acid (0, 2.5 and 5 mM), on different the morphological and physiological characteristics of garden cress exposed to chromium-VI stress (0, 1, and 10 mM). Results showed that some growth parameters, chlorophyll, carotenoids, and DPPH activity decre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Food Properties
سال: 2018
ISSN: 1094-2912,1532-2386
DOI: 10.1080/10942912.2018.1540989