Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits
نویسندگان
چکیده
منابع مشابه
Synthesis and Degradation of Rat Liver Lactate Dehydrogenase M4 HYBRIDIZATION IN THE PURIFICATION OF LACTATE DEHYDROGENASE
For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
VARIATIONS BY EPINEPHRINE OF HEPATIC AND SERUM AMINOTRANSFERASES AND LACTATE DEHYDROGENASE IN THE RAT
Incubation of rat hepatocytes with epinephrine inhibited alanine aminotransferase (ALT) (80%) and aspartate aminotransferase (AST) (53%) activities with no effect on lactate dehydrogenase (LDH) activity. Injection of epinephrine caused a progressive increase with time in hepatic LDH activity, being 52% at 24 h. Preinjection with propranolol eliminated the hormone effect and caused further ...
متن کاملSynthesis and degradation of rat liver lactate dehydrogenase M4. Hybridization in the purification of lactate dehydrogenase isoenzymes.
For the measurement of lactate dehydrogenase (LDH) isoenzyme biosynthesis a new method is presented which is based on the quantitative hybridization of isoenzyme MI with isoenzyme Hq. After reversible dissociation the formation of active LDH M4 was more rapid and occurred to a greater extent than the formation of LDH Hq. The formation of hybrid isoenzymes from the two subunits resulted in an in...
متن کامل[Purification of subunits from human chorionic gonadotropin and radioimmunoassay].
A crude hCG with an activity of about 3,000 IU per mg was purifed to 10,000--15,000 IU per mg of dry weight using Amberlite CG-50 chromatography combined with DEAE-Sephadex A-50 and Sephadex G-75. The alpha and beta subunits of hCG were prepared by urea-treatment of the hormone and isolated by DEAE-Sephadex A-50 chromatography. Further purification of the subunits was achieved by gel filtration...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1982
ISSN: 0264-6021
DOI: 10.1042/bj2050313