Purification and properties of Klebsiella pneumoniae heat-stable enterotoxin
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چکیده
منابع مشابه
Purification and properties of Klebsiella pneumoniae heat-stable enterotoxin.
The enterotoxic material in cell-free growth preparations of Klebsiella pneumoniae serotype 5 was purified by sequential ultrafiltration and gel filtration (GF) procedures and the fractions were assayed for enterotoxic activity by determining their ability to induce in vivo net water secretion in the rat jejunum. Whole-cell lysates were inactive. Anaerobic broth culture conditions yielded a 10-...
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The properties of a synthetically produced peptide composed of the same primary structure of 18 amino acids described for human Escherichia coli heat-stable enterotoxin were compared with those of purified heat-stable toxin obtained by bacterial growth. The dosage required to evoke fluid secretion in the suckling mouse and rat ligated ileal loop assays was the same for both toxins. The antigeni...
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A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity wa...
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A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V. cholerae non-O1 (strain A-5) by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatographies on SP-Sephadex C-50 and DEAE-Sephadex A-25, and high-pressure liqu...
متن کاملProduction and purification of heat-stable enterotoxin b from a porcine Escherichia coli strain.
Production of heat-stable enterotoxin b (STb) by porcine Escherichia coli strains belonging to serogroup O115 was evaluated in ligated intestinal segments of adult rats. The conditions for optimal production and detection of STb were studied by using the STb-producing strain 4247. As STb production was similar in complex Trypticase soy broth and minimal Davis medium, the latter was used for the...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1976
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.13.2.373-381.1976